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Home > Product > ELISA Kit > Human Interleukin-1 Receptor-associated Kinase 4, IRAK-4 ELISA Kit

Human Interleukin-1 Receptor-associated Kinase 4, IRAK-4 ELISA Kit

Human Interleukin-1 Receptor-associated Kinase 4, IRAK-4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.31ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 1.31ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin-1 Receptor-Associated Kinase 4

Code

E5506Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.31ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed: 17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.

UniProt accession

MASS(Da)

51,530

GeneID

51135

Synonyms

Interleukin-1 receptor-associated kinase 4;IRAK 4;IRAK4;IRAK-4;Renal carcinoma antigen NY-REN-64

Gene names

IRAK4

Research area

Immunology

Target protein

IRAK4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5506Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 2.03 2.121 2.085
2.212
240ng/L 1.474 1.457 1.421
1.44
120ng/L 0.839 0.891 0.855
0.944
60ng/L 0.536 0.521 0.485
0.506
30ng/L 0.233 0.247 0.211
0.26
0ng/L 0.035 0.036 0
0.037

Precision

We measured random samples of E5506Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 191.1 8.42 4.4
Intra-Assay 2 18 123.2 6.11 5
Intra-Assay 3 18 53 2.38 4.5

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E5506Hu

    $320.00

  • 96T

    E5506Hu

    $458.00

Add to Cart

Lead time: Within two weeks

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