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Home > Product > ELISA Kit > Human Interleukin-27 Subunit Beta, EBI3 ELISA Kit

Human Interleukin-27 Subunit Beta, EBI3 ELISA Kit

Human Interleukin-27 Subunit Beta, EBI3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.64ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.64ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin-27 Subunit Beta

Code

E5520Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.64ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.

UniProt accession

MASS(Da)

25,396

GeneID

10148

Synonyms

EBI 3;EBI3;EBV-induced gene 3 protein;Epstein-Barr virus-induced gene 3 protein;IL-27 subunit beta;IL-27B;Interleukin-27 subunit beta

Gene names

EBI3

Research area

Immunology

Target protein

EBI3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5520Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 1.98 2.133 2.08
2.286
600ng/L 1.336 1.378 1.326
1.419
300ng/L 0.717 0.704 0.652
0.691
150ng/L 0.375 0.391 0.339
0.407
75ng/L 0.118 0.129 0.077
0.14
0ng/L 0.05 0.052 0
0.054

Precision

We measured random samples of E5520Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 471.8 22.3 4.7
Intra-Assay 2 18 726 31.25 4.3
Intra-Assay 3 18 286.4 13.09 4.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E5520Hu

    $320.00

  • 96T

    E5520Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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