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Home > Product > ELISA Kit > Human Polycystin-1, PKD1 ELISA Kit

Human Polycystin-1, PKD1 ELISA Kit

Human Polycystin-1, PKD1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 8.96ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 8.96ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Polycystin-1

Code

E5896Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

8.96ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Component of a heteromeric calcium-permeable ion Channel formed by PKD1 and PKD2 that is activated by interaction between PKD1 and a Wnt family member, such as WNT3A and WNT9B (PubMed: 27214281). Both PKD1 and PKD2 are required for Channel activity (PubMed: 27214281). Involved in renal tubulogenesis (PubMed: 12482949). Involved in fluid-flow mechanosensation by the primary cilium in renal epithelium (By similarity). Acts as a regulator of cilium length, together with PKD2 (By similarity). The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling (By similarity). The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling (By similarity). May be an ion-Channel regulator. Involved in adhesive protein-protein and protein-carbohydrate interactions.

UniProt accession

MASS(Da)

462,529

GeneID

5310

Synonyms

Autosomal dominant polycystic kidney disease 1 protein;PC1;PKD 1;PKD1;Polycystin-1

Gene names

PKD1

Research area

Others

Target protein

PKD1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5896Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.287 2.289 2.23
2.29
1200ng/L 1.347 1.291 1.232
1.234
600ng/L 0.851 0.823 0.765
0.795
300ng/L 0.53 0.518 0.459
0.506
150ng/L 0.237 0.234 0.176
0.231
0ng/L 0.06 0.058 0
0.057

Precision

We measured random samples of E5896Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 213.9 14.52 6.8
Intra-Assay 2 18 1424.6 48.77 3.4
Intra-Assay 3 18 666.6 24.19 3.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E5896Hu

    $320.00

  • 96T

    E5896Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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