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Home > Product > ELISA Kit > Human Protein-glutamine Gamma-glutamyltransferase E, TGM3 ELISA Kit

Human Protein-glutamine Gamma-glutamyltransferase E, TGM3 ELISA Kit

Human Protein-glutamine Gamma-glutamyltransferase E, TGM3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.93ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.93ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Protein-Glutamine Gamma-Glutamyltransferase E

Code

E6022Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.93ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.

UniProt accession

MASS(Da)

76,632

GeneID

7053

Synonyms

Protein-glutamine gamma-glutamyltransferase E;Protein-glutamine gamma-glutamyltransferase E 27 kDa non-catalytic chain;Protein-glutamine gamma-glutamyltransferase E 50 kDa catalytic chain;TG(E;TGase E;TGase-3;TGE;TGM 3;TGM3;Transglutaminase E;Transglutaminase-3

Gene names

TGM3

Research area

Others

Target protein

TGM3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6022Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.128 2.158 2.117
2.187
1200ng/L 1.931 1.817 1.776
1.704
600ng/L 1.28 1.29 1.249
1.299
300ng/L 0.721 0.702 0.662
0.684
150ng/L 0.421 0.412 0.371
0.404
0ng/L 0.028 0.041 0
0.054

Precision

We measured random samples of E6022Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1116.2 82.45 7.4
Intra-Assay 2 18 289.1 14.79 5.1
Intra-Assay 3 18 572 39.66 6.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E6022Hu

    $320.00

  • 96T

    E6022Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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