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Home > Product > ELISA Kit > Human Sam Pointed Domain-containing Ets Transcription Factor, SPDEF ELISA Kit

Human Sam Pointed Domain-containing Ets Transcription Factor, SPDEF ELISA Kit

Human Sam Pointed Domain-containing Ets Transcription Factor, SPDEF ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 11.14ng/L

Detection range: 20-7000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 11.14ng/L

Detection range: 20-7000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Sam Pointed Domain-Containing Ets Transcription Factor

Code

E6117Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

11.14ng/L

Detection range

20-7000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.

UniProt accession

MASS(Da)

37,518

GeneID

25803

Synonyms

Prostate epithelium-specific Ets transcription factor;Prostate-derived Ets factor;Prostate-specific Ets;SAM pointed domain-containing Ets transcription factor;SPDEF

Gene names

SPDEF

Research area

Epigenetics and Nuclear Signaling

Target protein

SPDEF

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6117Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4000ng/L 2.452 2.533 2.487
2.614
2000ng/L 1.404 1.424 1.378
1.444
1000ng/L 0.757 0.826 0.78
0.895
500ng/L 0.509 0.475 0.429
0.441
250ng/L 0.23 0.237 0.191
0.243
0ng/L 0.048 0.046 0
0.044

Precision

We measured random samples of E6117Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 937.4 53.35 5.7
Intra-Assay 2 18 1698.2 59.37 3.5
Intra-Assay 3 18 799.3 42.1 5.3

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6117Hu

    $320.00

  • 96T

    E6117Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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