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Home > Product > ELISA Kit > Human Serine/Arginine-rich Splicing Factor 1, SFRS1 ELISA Kit

Human Serine/Arginine-rich Splicing Factor 1, SFRS1 ELISA Kit

Human Serine/Arginine-rich Splicing Factor 1, SFRS1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.31ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.31ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Serine/Arginine-Rich Splicing Factor 1

Code

E6143Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.31ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.

UniProt accession

MASS(Da)

27,745

GeneID

6426

Synonyms

Alternative-splicing factor 1;ASF-1;pre-mRNA-splicing factor SF2, P33 subunit;Serine/arginine-rich splicing factor 1;Splicing factor, arginine/serine-rich 1;SRSF 1;SRSF1

Gene names

SRSF1

Research area

Epigenetics and Nuclear Signaling

Target protein

SRSF1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6143Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.196 2.264 2.206
2.333
1200ng/L 1.552 1.491 1.432
1.429
600ng/L 0.911 0.885 0.827
0.859
300ng/L 0.492 0.461 0.402
0.429
150ng/L 0.253 0.252 0.194
0.251
0ng/L 0.052 0.058 0
0.064

Precision

We measured random samples of E6143Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 361.1 19.16 5.3
Intra-Assay 2 18 1028.3 37.54 3.7
Intra-Assay 3 18 539.8 29 5.4

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6143Hu

    $320.00

  • 96T

    E6143Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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