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Home > Product > ELISA Kit > Human Smoothened Homolog, SMO ELISA Kit

Human Smoothened Homolog, SMO ELISA Kit

Human Smoothened Homolog, SMO ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.25ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.25ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Smoothened Homolog

Code

E6185Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.25ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia (PubMed: 19592253). Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity).

UniProt accession

MASS(Da)

86,397

GeneID

6608

Synonyms

Protein Gx;SMO;Smoothened homolog

Gene names

SMO

Research area

Others

Target protein

SMO

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6185Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.167 2.156 2.104
2.145
800ng/L 1.68 1.609 1.557
1.538
400ng/L 0.967 0.974 0.922
0.981
200ng/L 0.669 0.691 0.639
0.714
100ng/L 0.403 0.401 0.348
0.398
0ng/L 0.059 0.052 0
0.045

Precision

We measured random samples of E6185Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1086.6 61.8 5.7
Intra-Assay 2 18 107.6 6.52 6.1
Intra-Assay 3 18 478.8 32.78 6.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E6185Hu

    $320.00

  • 96T

    E6185Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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