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Home > Product > ELISA Kit > Human Sphingosine 1-phosphate Receptor Type 3, S1P3 ELISA Kit

Human Sphingosine 1-phosphate Receptor Type 3, S1P3 ELISA Kit

Human Sphingosine 1-phosphate Receptor Type 3, S1P3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.66ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.66ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Sphingosine 1-Phosphate Receptor Type 3

Code

E6217Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.66ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.

UniProt accession

MASS(Da)

42,250

GeneID

1903

Synonyms

Endothelial differentiation G-protein coupled receptor 3;S1P receptor 3;S1P receptor Edg-3;S1P3;S1PR3;Sphingosine 1-phosphate receptor 3;Sphingosine 1-phosphate receptor Edg-3

Gene names

S1PR3

Research area

Signal Transduction

Target protein

S1PR3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6217Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.039 2.17 2.117
2.3
800ng/L 1.481 1.409 1.356
1.337
400ng/L 0.765 0.821 0.768
0.877
200ng/L 0.493 0.514 0.462
0.536
100ng/L 0.22 0.23 0.178
0.241
0ng/L 0.07 0.052 0
0.035

Precision

We measured random samples of E6217Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1211 46.28 3.8
Intra-Assay 2 18 204.3 9.69 4.7
Intra-Assay 3 18 454.8 27.56 6.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E6217Hu

    $320.00

  • 96T

    E6217Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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