Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Splicing Factor 3B Subunit 1, SF3B1 ELISA Kit

Human Splicing Factor 3B Subunit 1, SF3B1 ELISA Kit

Human Splicing Factor 3B Subunit 1, SF3B1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.97ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.97ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Splicing Factor 3B Subunit 1

Code

E6218Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.97ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed: 27720643). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed: 12234937). Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92 (By similarity). May also be involved in the assembly of the 'E' complex (PubMed: 10882114). Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed: 15146077).

UniProt accession

MASS(Da)

145,830

GeneID

23451

Synonyms

Pre-mRNA-splicing factor SF3b 155 kDa subunit;SAP 155;SF3B1;SF3b155;Spliceosome-associated protein 155;Splicing factor 3B subunit 1

Gene names

SF3B1

Research area

Others

Target protein

SF3B1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6218Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.319 2.184 2.124
2.049
1600ng/L 1.441 1.382 1.322
1.324
800ng/L 0.819 0.825 0.765
0.83
400ng/L 0.439 0.444 0.384
0.449
200ng/L 0.266 0.267 0.207
0.268
0ng/L 0.053 0.06 0
0.067

Precision

We measured random samples of E6218Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2367.6 105.32 4.4
Intra-Assay 2 18 1180.9 55.58 4.7
Intra-Assay 3 18 574 34.47 6

Protocols

Customer reviews and Q&As

No reviews
No reviews

Associated products

  • Size

    Code

    Price

  • 48T

    E6218Hu

    $320.00

  • 96T

    E6218Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up