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Home > Product > ELISA Kit > Human Spondin-1, SPON1 ELISA Kit

Human Spondin-1, SPON1 ELISA Kit

Human Spondin-1, SPON1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.028ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.028ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Spondin-1

Code

E6219Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.028ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.

UniProt accession

MASS(Da)

90,973

GeneID

10418

Synonyms

F-spondin;SPON 1;SPON1;Spondin-1;Vascular smooth muscle cell growth-promoting factor

Gene names

SPON1

Research area

Neuroscience

Target protein

SPON1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6219Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 1.973 1.982 1.939
1.991
6ng/mL 1.513 1.434 1.39
1.354
3ng/mL 0.564 0.616 0.573
0.668
1.5ng/mL 0.389 0.376 0.333
0.362
0.75ng/mL 0.132 0.152 0.109
0.172
0ng/mL 0.041 0.043 0
0.045

Precision

We measured random samples of E6219Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2.7 0.1 3.8
Intra-Assay 2 18 7.8 0.35 4.5
Intra-Assay 3 18 4.5 0.22 4.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E6219Hu

    $320.00

  • 96T

    E6219Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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