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Home > Product > ELISA Kit > Human Sterol Regulatory Element-binding Protein 2, SREBF2 ELISA Kit

Human Sterol Regulatory Element-binding Protein 2, SREBF2 ELISA Kit

Human Sterol Regulatory Element-binding Protein 2, SREBF2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.78ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.78ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Sterol Regulatory Element-Binding Protein 2

Code

E6225Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.78ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

[Sterol regulatory element-binding protein 2]: Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 2), which is embedded in the endoplasmic reticulum membrane (PubMed: 32322062). Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis (PubMed: 32322062). [Processed sterol regulatory element-binding protein 2]: Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis (PubMed: 12177166, PubMed: 32322062). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3') (PubMed: 7903453, PubMed: 12177166). Regulates transcription of genes related to cholesterol synthesis pathway (PubMed: 12177166, PubMed: 32322062).

UniProt accession

MASS(Da)

123,688

GeneID

6721

Synonyms

bHLHd2;Class D basic helix-loop-helix protein 2;Processed sterol regulatory element-binding protein 2;SREBF 2;SREBF2;SREBP-2;Sterol regulatory element-binding protein 2;Sterol regulatory element-binding transcription factor 2;Transcription factor SREBF2;

Gene names

SREBF2

Research area

Others

Target protein

SREBF2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6225Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.051 2.217 2.171
2.383
600ng/L 1.902 1.862 1.816
1.821
300ng/L 1.185 1.241 1.196
1.298
150ng/L 0.675 0.683 0.638
0.692
75ng/L 0.433 0.416 0.37
0.398
0ng/L 0.063 0.046 0
0.028

Precision

We measured random samples of E6225Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 417.7 21.03 5
Intra-Assay 2 18 105.9 5.8 5.5
Intra-Assay 3 18 299.3 20.52 6.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E6225Hu

    $320.00

  • 96T

    E6225Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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