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Home > Product > ELISA Kit > Horse Angiogenin, ANG ELISA Kit

Horse Angiogenin, ANG ELISA Kit

Horse Angiogenin, ANG ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 27.28ng/mL

Detection range: 50-10000ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Horse

Product Summary

Size: 96T,48T

Sensitivity: 27.28ng/mL

Detection range: 50-10000ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Horse

Product overview

Full product name

Angiogenin

Code

E0112HO

Assay type

Sandwich

Size

96T,48T

Sensitivity

27.28ng/mL

Detection range

50-10000ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Horse

Storage

2-8ºC

Assay time

1h 30m

Background

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs) (By similarity). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo (By similarity).

UniProt accession

MASS(Da)

16,410

GeneID

100034041

Synonyms

ANG;Angiogenin;Ribonuclease 5;RNase 5

Gene names

ANG

Research area

Signal Transduction; Epigenetics and Nuclear Signaling; Neuroscience; Cardiovascular; Cancer

Target protein

ANG

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0112HO are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6400ng/mL 1.9 2.03 1.988
2.16
3200ng/mL 1.06 1.064 1.022
1.068
1600ng/mL 0.549 0.525 0.483
0.5
800ng/mL 0.26 0.225 0.182
0.19
400ng/mL 0.169 0.148 0.106
0.128
0ng/mL 0.033 0.042 0
0.051

Precision

We measured random samples of E0112HO within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2298.2 111.51 4.9
Intra-Assay 2 18 4188.6 121.16 2.9
Intra-Assay 3 18 1419.5 59.03 4.2

Protocols

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  • Size

    Code

    Price

  • 96T

    E0112HO

    $498.00

  • 48T

    E0112HO

    $360.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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