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Home > Product > ELISA Kit > Bovine Endothelin 1, ET-1 ELISA Kit

Bovine Endothelin 1, ET-1 ELISA Kit

Bovine Endothelin 1, ET-1 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 1.19ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 1.19ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Endothelin 1

Code

E0122Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

1.19ng/L

Detection range

2-600ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Endothelins are endothelium-derived vasoconstrictor Peptides (By similarity). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (By similarity).

UniProt accession

MASS(Da)

22,977

GeneID

281137

Synonyms

Big endothelin-1;EDN 1;EDN1;Endothelin-1;ET-1;PPET1;Preproendothelin-1

Gene names

EDN1

Research area

Signal Transduction

Target protein

EDN1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0122Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
320ng/L 2.175 2.136 2.08
2.097
160ng/L 1.398 1.404 1.348
1.41
80ng/L 0.712 0.74 0.684
0.767
40ng/L 0.459 0.447 0.392
0.436
20ng/L 0.269 0.252 0.196
0.235
0ng/L 0.063 0.056 0
0.049

Precision

We measured random samples of E0122Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 101.4 6.29 6.2
Intra-Assay 2 18 153.7 6.45 4.2
Intra-Assay 3 18 50.3 2.44 4.8

Protocols

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  • Size

    Code

    Price

  • 96T

    E0122Bo

    $458.00

  • 48T

    E0122Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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