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Home > Product > ELISA Kit > Horse Hepatocyte Growth Factor Receptor, MET ELISA Kit

Horse Hepatocyte Growth Factor Receptor, MET ELISA Kit

Horse Hepatocyte Growth Factor Receptor, MET ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 5.29ng/ml

Detection range: 10-2000ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Horse

Product Summary

Size: 96T,48T

Sensitivity: 5.29ng/ml

Detection range: 10-2000ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Horse

Product overview

Full product name

Hepatocyte Growth Factor Receptor

Code

E0122HO

Assay type

Sandwich

Size

96T,48T

Sensitivity

5.29ng/ml

Detection range

10-2000ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Horse

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells (By similarity).

UniProt accession

MASS(Da)

154,561

GeneID

100056013

Synonyms

Hepatocyte growth factor receptor;HGF receptor;HGF/SF receptor;MET;Proto-oncogene c-Met;Scatter factor receptor;SF receptor;Tyrosine-protein kinase Met

Gene names

MET

Research area

Others

Target protein

MET

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0122HO are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1280ng/ml 2.2 2.183 2.149
2.167
640ng/ml 1.59 1.577 1.542
1.564
320ng/ml 1.182 1.084 1.049
0.986
160ng/ml 0.703 0.7 0.666
0.698
80ng/ml 0.445 0.45 0.415
0.454
0ng/ml 0.037 0.034 0
0.032

Precision

We measured random samples of E0122HO within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 545.7 26.31 4.8
Intra-Assay 2 18 131 8.79 6.7
Intra-Assay 3 18 324.7 24.38 7.5

Protocols

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  • Size

    Code

    Price

  • 96T

    E0122HO

    $498.00

  • 48T

    E0122HO

    $360.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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