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Home > Product > ELISA Kit > Horse Leukocyte Elastase Inhibitor, SERPINB1 ELISA Kit

Horse Leukocyte Elastase Inhibitor, SERPINB1 ELISA Kit

Horse Leukocyte Elastase Inhibitor, SERPINB1 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 10.11ng/mL

Detection range: 20-4500ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Horse

Product Summary

Size: 96T,48T

Sensitivity: 10.11ng/mL

Detection range: 20-4500ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Horse

Product overview

Full product name

Leukocyte Elastase Inhibitor

Code

E0131HO

Assay type

Sandwich

Size

96T,48T

Sensitivity

10.11ng/mL

Detection range

20-4500ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Horse

Storage

2-8ºC

Assay time

1h 30m

Background

Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis. Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Acts also as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity. During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation. When secreted, promotes the proliferation of beta-cells via its protease inhibitory function.

UniProt accession

MASS(Da)

43,006

GeneID

100049797

Synonyms

LEI;Leukocyte elastase inhibitor;Serpin B1;SERPINB 1;SERPINB1

Gene names

SERPINB1

Research area

Others

Target protein

SERPINB1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0131HO are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/mL 1.929 1.823 1.79
1.718
1200ng/mL 0.868 0.923 0.889
0.978
600ng/mL 0.636 0.636 0.602
0.635
300ng/mL 0.192 0.223 0.189
0.254
150ng/mL 0.07 0.105 0.071
0.14
0ng/mL 0.031 0.034 0
0.036

Precision

We measured random samples of E0131HO within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1483.1 90.02 6.1
Intra-Assay 2 18 1817.4 62.11 3.4
Intra-Assay 3 18 414.8 21.56 5.2

Protocols

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  • Size

    Code

    Price

  • 96T

    E0131HO

    $498.00

  • 48T

    E0131HO

    $360.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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