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Home > Product > ELISA Kit > Human Thyroglobulin, TG ELISA Kit

Human Thyroglobulin, TG ELISA Kit

Human Thyroglobulin, TG ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.021ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.021ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Thyroglobulin

Code

E0008Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.021ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3) (PubMed: 32025030, PubMed: 17532758). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling in the thyroid follicle lumen (PubMed: 32025030). Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream (PubMed: 32025030). One dimer produces 7 thyroid hormone molecules (PubMed: 32025030).

UniProt accession

MASS(Da)

304,790

GeneID

7038

Synonyms

Tg;Thyroglobulin

Gene names

TG

Research area

Metabolism

Target protein

TG

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0008Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.279 2.175 2.122
2.072
8ng/ml 1.936 1.87 1.816
1.804
4ng/ml 1.162 1.221 1.168
1.281
2ng/ml 0.61 0.633 0.58
0.656
1ng/ml 0.421 0.396 0.343
0.372
0ng/ml 0.058 0.053 0
0.049

Precision

We measured random samples of E0008Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.1 0.05 4.5
Intra-Assay 2 18 7.8 0.45 5.8
Intra-Assay 3 18 2.3 0.1 4.4

Protocols

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  • Size

    Code

    Price

  • 96T

    E0008Hu

    $458.00

  • 48T

    E0008Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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