Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Prolyl 3-hydroxylase 1, P3H1 ELISA Kit

Human Prolyl 3-hydroxylase 1, P3H1 ELISA Kit

Human Prolyl 3-hydroxylase 1, P3H1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.011ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.011ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Prolyl 3-Hydroxylase 1

Code

E7297Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.011ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts.

UniProt accession

MASS(Da)

83,394

GeneID

64175

Synonyms

Growth suppressor 1;Leprecan-1;Leucine- and proline-enriched proteoglycan 1;P3H1;Prolyl 3-hydroxylase 1

Gene names

P3H1

Research area

Signal Transduction

Target protein

P3H1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E7297Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6.4ng/ml 2.046 1.955 1.908
1.864
3.2ng/ml 1.107 1.078 1.031
1.049
1.6ng/ml 0.51 0.507 0.46
0.503
0.8ng/ml 0.174 0.214 0.168
0.254
0.4ng/ml 0.166 0.146 0.099
0.125
0ng/ml 0.036 0.047 0
0.057

Precision

We measured random samples of E7297Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.3 0.17 3.2
Intra-Assay 2 18 2.4 0.1 4
Intra-Assay 3 18 1.7 0.08 4.9

Protocols

Customer reviews and Q&As

No reviews
No reviews

Associated products

  • Size

    Code

    Price

  • 48T

    E7297Hu

    $320.00

  • 96T

    E7297Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up