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Home > Product > ELISA Kit > Human Protein Phosphatase, Mg2+, Mn2+ Dependent 1D, PPM1D ELISA Kit

Human Protein Phosphatase, Mg2+, Mn2+ Dependent 1D, PPM1D ELISA Kit

Human Protein Phosphatase, Mg2+, Mn2+ Dependent 1D, PPM1D ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Protein Phosphatase, Mg2+/Mn2+ Dependent 1D

Code

E7328Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.019ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in the negative regulation of p53 expression (PubMed: 23242139). Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins (PubMed: 15870257, PubMed: 16311512). Mediates MAPK14 dephosphorylation and inactivation (PubMed: 21283629). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (By similarity).

UniProt accession

MASS(Da)

66,675

GeneID

8493

Synonyms

p53-induced protein phosphatase 1;PP2C-delta;PPM 1D;PPM1D;Protein phosphatase 1D;Protein phosphatase 2C isoform delta;Protein phosphatase magnesium-dependent 1 delta

Gene names

PPM1D

Research area

Signal Transduction

Target protein

PPM1D

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E7328Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 1.9 1.936 1.882
1.972
4ng/ml 1.138 1.124 1.069
1.109
2ng/ml 0.506 0.512 0.458
0.518
1ng/ml 0.299 0.231 0.176
0.164
0.5ng/ml 0.071 0.114 0.059
0.157
0ng/ml 0.056 0.055 0
0.053

Precision

We measured random samples of E7328Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.3 0.19 4.3
Intra-Assay 2 18 7.3 0.28 3.9
Intra-Assay 3 18 1.8 0.07 3.8

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E7328Hu

    $320.00

  • 96T

    E7328Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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