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Home > Product > ELISA Kit > Mouse Angiotensin Converting Enzyme, ACE ELISA Kit

Mouse Angiotensin Converting Enzyme, ACE ELISA Kit

Mouse Angiotensin Converting Enzyme, ACE ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.28ng/mL

Detection range: 0.5-200ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.28ng/mL

Detection range: 0.5-200ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Angiotensin Converting Enzyme

Code

E0016Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.28ng/mL

Detection range

0.5-200ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.

UniProt accession

MASS(Da)

150,918

GeneID

11421

Synonyms

ACE;Angiotensin-converting enzyme;Angiotensin-converting enzyme, soluble form;CD antigen CD143;Dipeptidyl carboxypeptidase I;Kininase II

Gene names

ACE

Research area

Cardiovascular; Signal Transduction; Cell Biology; Cardiovascular; Cancer; Metabolism

Target protein

ACE

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0016Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
120ng/mL 2.369 2.482 2.441
2.595
60ng/mL 1.367 1.435 1.394
1.504
30ng/mL 0.822 0.807 0.766
0.791
15ng/mL 0.435 0.482 0.441
0.529
7.5ng/mL 0.275 0.232 0.191
0.189
0ng/mL 0.032 0.041 0
0.05

Precision

We measured random samples of E0016Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 11.5 0.63 5.5
Intra-Assay 2 18 70.7 2.76 3.9
Intra-Assay 3 18 35 1.55 4.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E0016Mo

    $320.00

  • 96T

    E0016Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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