Product overview
Full product name
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Background
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed: 25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
UniProt accession
MASS（Da）
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Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0117Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 640ng/L | 2.342 | 2.349 | 2.314 |
| 2.355 | |||
| 320ng/L | 1.414 | 1.437 | 1.402 |
| 1.46 | |||
| 160ng/L | 0.826 | 0.777 | 0.742 |
| 0.727 | |||
| 80ng/L | 0.481 | 0.483 | 0.448 |
| 0.486 | |||
| 40ng/L | 0.273 | 0.277 | 0.242 |
| 0.28 | |||
| 0ng/L | 0.036 | 0.035 | 0 |
| 0.034 |
Precision
We measured random samples of E0117Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 130.1 | 8.28 | 6.4 |
| Intra-Assay | 2 | 18 | 305.7 | 11.44 | 3.7 |
| Intra-Assay | 3 | 18 | 169.6 | 8.45 | 5 |
Protocols
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