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Home > Product > ELISA Kit > Mouse Peroxisome Proliferators Activator Receptors Alpha, PPAR-A ELISA Kit

Mouse Peroxisome Proliferators Activator Receptors Alpha, PPAR-A ELISA Kit

Mouse Peroxisome Proliferators Activator Receptors Alpha, PPAR-A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.034ng/mL

Detection range: 0.06-18ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.034ng/mL

Detection range: 0.06-18ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Peroxisome Proliferators Activator Receptors Alpha

Code

E0146Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.034ng/mL

Detection range

0.06-18ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.

UniProt accession

MASS(Da)

52,347

GeneID

19013

Synonyms

Nuclear receptor subfamily 1 group C member 1;Peroxisome proliferator-activated receptor alpha;PPARA;PPAR-alpha

Gene names

PPARA

Research area

Others

Target protein

PPARA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0146Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
9.6ng/mL 2.096 2.179 2.145
2.263
4.8ng/mL 1.317 1.41 1.376
1.504
2.4ng/mL 0.802 0.839 0.804
0.876
1.2ng/mL 0.44 0.461 0.427
0.483
0.6ng/mL 0.251 0.238 0.204
0.225
0ng/mL 0.036 0.035 0
0.033

Precision

We measured random samples of E0146Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.7 0.09 5.6
Intra-Assay 2 18 3.5 0.23 6.5
Intra-Assay 3 18 6.1 0.26 4.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E0146Mo

    $320.00

  • 96T

    E0146Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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