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Home > Product > ELISA Kit > Mouse Growth Differentiation Factor 15, GDF15 ELISA Kit

Mouse Growth Differentiation Factor 15, GDF15 ELISA Kit

Mouse Growth Differentiation Factor 15, GDF15 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.52ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 1.52ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Growth Differentiation Factor 15

Code

E0224Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.52ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which contitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions (PubMed: 28953886, PubMed: 28846097, PubMed: 28846099, PubMed: 28572090, PubMed: 23468844, PubMed: 29046435). On hepatocytes, inhibits growth hormone signaling (PubMed: 28572090).

UniProt accession

MASS(Da)

33,225

GeneID

23886

Synonyms

GDF 15;GDF15;GDF-15;Growth/differentiation factor 15;Macrophage inhibitory cytokine 1;MIC-1

Gene names

GDF15

Research area

Signal Transduction

Target protein

GDF15

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0224Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 2.232 2.189 2.119
2.146
240ng/L 1.447 1.409 1.339
1.371
120ng/L 0.771 0.759 0.689
0.748
60ng/L 0.474 0.492 0.421
0.509
30ng/L 0.246 0.238 0.168
0.231
0ng/L 0.068 0.07 0
0.072

Precision

We measured random samples of E0224Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 358.7 17.9 5
Intra-Assay 2 18 60.5 4.21 7
Intra-Assay 3 18 189.4 10.68 5.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E0224Mo

    $320.00

  • 96T

    E0224Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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