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Home > Product > ELISA Kit > Mouse Tumor Necrosis Factor Β, TNF-B ELISA Kit

Mouse Tumor Necrosis Factor Β, TNF-B ELISA Kit

Mouse Tumor Necrosis Factor Β, TNF-B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.26ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.26ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Tumor Necrosis Factor beta

Code

E0255Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.26ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (By similarity). In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.

UniProt accession

MASS(Da)

21,999

GeneID

16992

Synonyms

LT-alpha;Lymphotoxin-alpha;TNF B;TNF beta;TNFB;TNF-beta;Tumor necrosis factor ligand superfamily member 1

Gene names

TNFB

Research area

Immunology; Signal Transduction; Cancer

Target protein

TNFB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0255Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.102 2.194 2.155
2.285
600ng/L 1.219 1.218 1.18
1.218
300ng/L 0.629 0.67 0.632
0.711
150ng/L 0.468 0.43 0.392
0.392
75ng/L 0.235 0.214 0.176
0.193
0ng/L 0.048 0.038 0
0.028

Precision

We measured random samples of E0255Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 467.1 25.58 5.5
Intra-Assay 2 18 729 23.85 3.3
Intra-Assay 3 18 250.1 17.63 7

Protocols

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  • Size

    Code

    Price

  • 48T

    E0255Mo

    $320.00

  • 96T

    E0255Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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