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Home > Product > ELISA Kit > Mouse Hypoxia-inducible Factor 1Α, HIF-1A ELISA Kit

Mouse Hypoxia-inducible Factor 1Α, HIF-1A ELISA Kit

Mouse Hypoxia-inducible Factor 1Α, HIF-1A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.153ng/L

Detection range: 0.3-90ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.153ng/L

Detection range: 0.3-90ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Hypoxia-Inducible Factor 1 alpha

Code

E0294Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.153ng/L

Detection range

0.3-90ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed: 26245371). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (By similarity).

UniProt accession

MASS(Da)

93,516

GeneID

15251

Synonyms

ARNT-interacting protein;HIF 1A;HIF1A;HIF1-alpha;HIF-1-alpha;Hypoxia-inducible factor 1-alpha

Gene names

HIF1A

Research area

Epigenetics and Nuclear Signaling

Target protein

HIF1A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0294Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/L 2.138 2.162 2.123
2.185
24ng/L 1.943 1.876 1.837
1.809
12ng/L 1.173 1.156 1.117
1.14
6ng/L 0.661 0.645 0.606
0.629
3ng/L 0.359 0.353 0.313
0.346
0ng/L 0.046 0.039 0
0.032

Precision

We measured random samples of E0294Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 14.2 0.53 3.7
Intra-Assay 2 18 4.6 0.19 4.2
Intra-Assay 3 18 11.3 0.55 4.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E0294Mo

    $320.00

  • 96T

    E0294Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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