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Home > Product > ELISA Kit > Mouse Osteopontin, OPN ELISA Kit

Mouse Osteopontin, OPN ELISA Kit

Mouse Osteopontin, OPN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.023ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.023ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Osteopontin

Code

E0322Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.023ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

UniProt accession

MASS(Da)

32,459

GeneID

20750

Synonyms

2AR;Bone sialoprotein 1;Calcium oxalate crystal growth inhibitor protein;Early T-lymphocyte activation 1 protein;Minopontin;Osteopontin;Secreted phosphoprotein 1;SPP 1;SPP1;SPP-1

Gene names

SPP1

Research area

Signal Transduction

Target protein

SPP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0322Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.557 2.565 2.519
2.573
4ng/ml 1.413 1.387 1.341
1.362
2ng/ml 0.759 0.743 0.697
0.726
1ng/ml 0.479 0.495 0.449
0.51
0.5ng/ml 0.272 0.266 0.221
0.26
0ng/ml 0.052 0.046 0
0.04

Precision

We measured random samples of E0322Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.5 0.15 2.6
Intra-Assay 2 18 1.3 0.08 6.7
Intra-Assay 3 18 2.5 0.12 4.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E0322Mo

    $320.00

  • 96T

    E0322Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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