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Home > Product > ELISA Kit > Mouse Inducible Nitric Oxide Synthase, INOS ELISA Kit

Mouse Inducible Nitric Oxide Synthase, INOS ELISA Kit

Mouse Inducible Nitric Oxide Synthase, INOS ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.16ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.16ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Inducible Nitric Oxide Synthase

Code

E0386Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.16ng/ml

Detection range

0.3-90ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed: 7503239). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (PubMed: 16373578). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (By similarity). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (By similarity).

UniProt accession

MASS(Da)

130,575

GeneID

18126

Synonyms

Inducible NO Synthase;Inducible NOS;iNOS;MAC-NOS;Macrophage NOS;Nitric oxide Synthase, inducible;NOS 2;NOS type II;NOS2;Peptidyl-cysteine S-nitrosylase NOS2

Gene names

NOS2

Research area

Neuroscience

Target protein

NOS2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0386Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/ml 2.282 2.279 2.223
2.276
24ng/ml 1.893 1.798 1.743
1.703
12ng/ml 1.286 1.265 1.21
1.245
6ng/ml 0.609 0.634 0.578
0.658
3ng/ml 0.399 0.387 0.332
0.375
0ng/ml 0.059 0.055 0
0.052

Precision

We measured random samples of E0386Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 21.1 1.26 6
Intra-Assay 2 18 9.8 0.63 6.5
Intra-Assay 3 18 4.9 0.23 4.7

Protocols

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  • Size

    Code

    Price

  • 48T

    E0386Mo

    $320.00

  • 96T

    E0386Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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