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Home > Product > ELISA Kit > Mouse Complement Factor H, CFH ELISA Kit

Mouse Complement Factor H, CFH ELISA Kit

Mouse Complement Factor H, CFH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.76ng/mL

Detection range: 20-4500ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.76ng/mL

Detection range: 20-4500ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Complement Factor H

Code

E0592Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.76ng/mL

Detection range

20-4500ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed.

UniProt accession

MASS(Da)

139,138

GeneID

12628

Synonyms

CFH;Complement factor H;Protein beta-1-H

Gene names

CFH

Research area

Immunology

Target protein

CFH

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0592Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/mL 2.64 2.515 2.455
2.389
1200ng/mL 1.451 1.438 1.377
1.424
600ng/mL 0.765 0.801 0.741
0.838
300ng/mL 0.429 0.462 0.402
0.495
150ng/mL 0.189 0.207 0.147
0.225
0ng/mL 0.059 0.06 0
0.061

Precision

We measured random samples of E0592Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1446.2 49.27 3.4
Intra-Assay 2 18 506.4 31.21 6.2
Intra-Assay 3 18 867.5 36.25 4.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E0592Mo

    $320.00

  • 96T

    E0592Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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