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Home > Product > ELISA Kit > Mouse Interleukin 21, IL-21 ELISA Kit

Mouse Interleukin 21, IL-21 ELISA Kit

Mouse Interleukin 21, IL-21 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.55ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 4.55ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Interleukin 21

Code

E0614Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.55ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (PubMed: 15100251). Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (PubMed: 23045607). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15 (PubMed: 11970879). May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (By similarity). During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (PubMed: 12893770).

UniProt accession

MASS(Da)

16,811

GeneID

60505

Synonyms

IL 21;IL21;IL-21;Interleukin-21

Gene names

IL21

Research area

Immunology; Cancer

Target protein

IL21

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0614Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.505 2.532 2.48
2.559
600ng/L 1.57 1.579 1.528
1.588
300ng/L 0.82 0.814 0.762
0.807
150ng/L 0.437 0.414 0.363
0.391
75ng/L 0.224 0.245 0.193
0.265
0ng/L 0.047 0.051 0
0.056

Precision

We measured random samples of E0614Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 484.8 12.34 2.5
Intra-Assay 2 18 279.9 12.25 4.4
Intra-Assay 3 18 235.4 11.32 4.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E0614Mo

    $320.00

  • 96T

    E0614Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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