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Home > Product > ELISA Kit > Mouse Leukemia Inhibitory Factor, LIF ELISA Kit

Mouse Leukemia Inhibitory Factor, LIF ELISA Kit

Mouse Leukemia Inhibitory Factor, LIF ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.046ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.046ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Leukemia Inhibitory Factor

Code

E0645Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.046ng/mL

Detection range

0.1-40ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

UniProt accession

MASS(Da)

22,287

GeneID

16878

Synonyms

D factor;Differentiation-stimulating factor;Leukemia inhibitory factor;LIF

Gene names

LIF

Research area

Cancer

Target protein

LIF

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0645Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/mL 2.195 2.199 2.149
2.203
12ng/mL 1.654 1.616 1.566
1.579
6ng/mL 1.114 1.045 0.995
0.976
3ng/mL 0.724 0.75 0.7
0.777
1.5ng/mL 0.476 0.466 0.417
0.457
0ng/mL 0.038 0.05 0
0.062

Precision

We measured random samples of E0645Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.2 0.41 7.9
Intra-Assay 2 18 14.5 0.74 5.1
Intra-Assay 3 18 1.2 0.09 7.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E0645Mo

    $320.00

  • 96T

    E0645Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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