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Home > Product > ELISA Kit > Mouse Growth Differentiation Factor 5, GDF5 ELISA Kit

Mouse Growth Differentiation Factor 5, GDF5 ELISA Kit

Mouse Growth Differentiation Factor 5, GDF5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.15ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.15ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Growth Differentiation Factor 5

Code

E0804Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.15ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (By similarity). Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (By similarity). Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (PubMed: 24076600). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (By similarity).

UniProt accession

MASS(Da)

54,895

GeneID

14563

Synonyms

BMP-14;Bone morphogenetic protein 14;GDF 5;GDF5;GDF-5;Growth/differentiation factor 5

Gene names

GDF5

Research area

Others

Target protein

GDF5

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0804Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.123 2.243 2.199
2.364
1200ng/L 1.404 1.265 1.221
1.127
600ng/L 0.787 0.768 0.723
0.749
300ng/L 0.412 0.417 0.372
0.421
150ng/L 0.222 0.204 0.159
0.186
0ng/L 0.043 0.045 0
0.047

Precision

We measured random samples of E0804Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 268.2 15.85 5.9
Intra-Assay 2 18 1118.4 56.05 5
Intra-Assay 3 18 1536.5 45.05 2.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E0804Mo

    $320.00

  • 96T

    E0804Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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