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Home > Product > ELISA Kit > Mouse Cyclin-dependent Kinase 4, CDK-4 ELISA Kit

Mouse Cyclin-dependent Kinase 4, CDK-4 ELISA Kit

Mouse Cyclin-dependent Kinase 4, CDK-4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.047ng/ml

Detection range: 0.1-50ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.047ng/ml

Detection range: 0.1-50ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Cyclin-Dependent Kinase 4

Code

E0965Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.047ng/ml

Detection range

0.1-50ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

UniProt accession

MASS(Da)

33,751

GeneID

12567

Synonyms

CDK 4;CDK4;Cell division protein kinase 4;CRK3;Cyclin-dependent kinase 4;PSK-J3

Gene names

CDK4

Research area

Cell Biology

Target protein

CDK4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0965Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
28ng/ml 2.253 2.237 2.183
2.221
14ng/ml 1.609 1.565 1.511
1.521
7ng/ml 1.124 1.154 1.1
1.184
3.5ng/ml 0.745 0.713 0.659
0.681
1.75ng/ml 0.482 0.464 0.41
0.447
0ng/ml 0.048 0.054 0
0.06

Precision

We measured random samples of E0965Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 12.3 0.36 2.9
Intra-Assay 2 18 4.9 0.37 7.5
Intra-Assay 3 18 1.9 0.15 7.7

Protocols

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  • Size

    Code

    Price

  • 48T

    E0965Mo

    $320.00

  • 96T

    E0965Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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