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Home > Product > ELISA Kit > Mouse Angiopoietin-like Protein 3, ANGPTL3 ELISA Kit

Mouse Angiopoietin-like Protein 3, ANGPTL3 ELISA Kit

Mouse Angiopoietin-like Protein 3, ANGPTL3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.62ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.62ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Angiopoietin-Like Protein 3

Code

E1107Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.62ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism (PubMed: 11788823, PubMed: 12671033). Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake (PubMed: 26305978). Has a stimulatory effect on plasma triglycerides (TG), which is achieved by suppressing plasma TG clearance via inhibition of LPL activity; the function seems to be specific for the feeding conditions. The inhibition of LPL activity appears to be an indirect mechanism involving recruitment of proprotein convertases PCSK6 and FURIN to LPL leading to cleavage and dissociation of LPL from the cell surface; the function does not require ANGPTL3 proteolytic cleavage but seems to be mediated by the N-terminal domain, and is not inhibited by GPIHBP1 (PubMed: 12909640, PubMed: 16081640, PubMed: 20581395). Can inhibit endothelial lipase, causing increased plasma levels of high density lipoprotein (HDL) cholesterol and phospholipids; the cleaved N-terminal domain is more efficient than the uncleaved proprotein (PubMed: 17681148). Can bind to adipocytes to activate lipolysis, releasing free fatty acids and glycerol (By similarity). Suppresses LPL specifically in oxidative tissues which is required to route very low density lipoprotein (VLDL)-TG to white adipose tissue (WAT) for storage in response to food; the function may involve cooperation with circulating, liver-derived ANGPTL8 and ANGPTL4 expression in WAT (PubMed: 26305978). Contributes to lower plasma levels of low density lipoprotein (LDL)-cholesterol by a mechanism that is independent of the canonical pathway implicating APOE and LDLR (PubMed: 25954050). May stimulate hypothalamic LPL activity (PubMed: 25338813). Involved in angiogenesis (PubMed: 11877390). Binds to endothelial cells via integrin alpha-V/beta-3 (ITGAV:ITGB3), activates FAK, MAPK and Akt signaling pathways and induces cell adhesion and cell migration (By similarity). May increase the motility of podocytes. Secreted from podocytes, may modulate properties of glomerular endothelial cells involving integrin alpha-V/beta-3 and Akt signaling (By similarity). May induce actin filament rearrangements in podocytes implicating integrin alpha-V/beta-3 and Rac1 activation (PubMed: 20633534, PubMed: 24294595, PubMed: 25710887). Binds to hematopoietic stem cells (HSC) and is involved in the regulation of HSC activity probably implicating down-regulation of IKZF1/IKAROS (PubMed: 20959605).

UniProt accession

MASS(Da)

52,543

GeneID

30924

Synonyms

Angiopoietin-like protein 3;Angiopoietin-related protein 3;ANGPTL 3;ANGPTL3;ANGPTL3(17-224

Gene names

ANGPTL3

Research area

Cardiovascular

Target protein

ANGPTL3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1107Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.112 2.19 2.131
2.269
1200ng/L 1.384 1.315 1.256
1.246
600ng/L 0.833 0.767 0.709
0.702
300ng/L 0.493 0.481 0.423
0.469
150ng/L 0.252 0.281 0.223
0.311
0ng/L 0.078 0.059 0
0.039

Precision

We measured random samples of E1107Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 586.4 37.7 6.4
Intra-Assay 2 18 1843.8 71.68 3.9
Intra-Assay 3 18 378.2 27.82 7.4

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E1107Mo

    $320.00

  • 96T

    E1107Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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