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Home > Product > ELISA Kit > Mouse Bactericidal Permeability-increasing Protein, BPI ELISA Kit

Mouse Bactericidal Permeability-increasing Protein, BPI ELISA Kit

Mouse Bactericidal Permeability-increasing Protein, BPI ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.145ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.145ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Bactericidal Permeability-Increasing Protein

Code

E1140Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.145ng/mL

Detection range

0.3-90ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope.

UniProt accession

MASS(Da)

53,940

GeneID

329547

Synonyms

Bactericidal permeability-increasing protein;BPI

Gene names

BPI

Research area

Immunology

Target protein

BPI

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1140Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/mL 2.109 1.994 1.947
1.879
24ng/mL 1.142 1.099 1.052
1.055
12ng/mL 0.48 0.465 0.418
0.45
6ng/mL 0.215 0.263 0.216
0.312
3ng/mL 0.149 0.117 0.07
0.085
0ng/mL 0.063 0.047 0
0.031

Precision

We measured random samples of E1140Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 37.4 1.43 3.8
Intra-Assay 2 18 9.1 0.33 3.6
Intra-Assay 3 18 20.4 0.78 3.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E1140Mo

    $320.00

  • 96T

    E1140Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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