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Home > Product > ELISA Kit > Mouse Kisspeptin 1, KISS-1 ELISA Kit

Mouse Kisspeptin 1, KISS-1 ELISA Kit

Mouse Kisspeptin 1, KISS-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.49ng/L

Detection range: 10-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.49ng/L

Detection range: 10-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Kisspeptin 1

Code

E1144Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.49ng/L

Detection range

10-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Metastasis suppressor protein. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated Peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Intracerebroventricular administration induces an increase in serum LH and FSH levels in prepubertal male and female as well as in adult animals.

UniProt accession

MASS(Da)

14,117

GeneID

280287

Synonyms

KISS 1;KISS1;Kisspeptin-1;Kisspeptin-10;Kisspeptin-52;Metastasis-suppressor KiSS-1;Metastin;Metastin45-54

Gene names

KISS1

Research area

Others

Target protein

KISS1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1144Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.212 2.131 2.072
2.05
800ng/L 1.185 1.245 1.186
1.306
400ng/L 0.626 0.634 0.575
0.642
200ng/L 0.449 0.45 0.39
0.451
100ng/L 0.245 0.249 0.189
0.253
0ng/L 0.059 0.06 0
0.06

Precision

We measured random samples of E1144Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 363.3 23.33 6.4
Intra-Assay 2 18 678.6 33.43 4.9
Intra-Assay 3 18 1025.8 32.85 3.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E1144Mo

    $320.00

  • 96T

    E1144Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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