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Home > Product > ELISA Kit > Mouse Cytochrome P450 2E1, CYP2E1 ELISA Kit

Mouse Cytochrome P450 2E1, CYP2E1 ELISA Kit

Mouse Cytochrome P450 2E1, CYP2E1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.093ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.093ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Cytochrome P450 2E1

Code

E1147Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.093ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.

UniProt accession

MASS(Da)

56,805

GeneID

13106

Synonyms

4-nitrophenol 2-hydroxylase;CYP 2E1;CYP2E1;CYPIIE1;Cytochrome P450 2E1;Cytochrome P450-ALC;Cytochrome P450-J

Gene names

CYP2E1

Research area

Cardiovascular

Target protein

CYP2E1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1147Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.084 2.139 2.069
2.194
16ng/ml 1.517 1.511 1.442
1.505
8ng/ml 0.819 0.863 0.793
0.906
4ng/ml 0.405 0.464 0.394
0.523
2ng/ml 0.25 0.24 0.171
0.231
0ng/ml 0.068 0.07 0
0.072

Precision

We measured random samples of E1147Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 15.6 0.66 4.2
Intra-Assay 2 18 9.8 0.39 4
Intra-Assay 3 18 4.1 0.21 5

Protocols

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  • Size

    Code

    Price

  • 48T

    E1147Mo

    $320.00

  • 96T

    E1147Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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