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Home > Product > ELISA Kit > Mouse Insulin Receptor Substrate 1, IRS-1 ELISA Kit

Mouse Insulin Receptor Substrate 1, IRS-1 ELISA Kit

Mouse Insulin Receptor Substrate 1, IRS-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.031ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.031ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Insulin Receptor Substrate 1

Code

E1163Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.031ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).

UniProt accession

MASS(Da)

130,723

GeneID

16367

Synonyms

Insulin receptor substrate 1;IRS 1;IRS1;IRS-1

Gene names

IRS1

Research area

Others

Target protein

IRS1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1163Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.364 2.339 2.306
2.314
8ng/ml 1.412 1.38 1.347
1.348
4ng/ml 0.684 0.643 0.61
0.601
2ng/ml 0.367 0.373 0.34
0.38
1ng/ml 0.157 0.152 0.119
0.146
0ng/ml 0.03 0.033 0
0.036

Precision

We measured random samples of E1163Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.5 0.21 3.9
Intra-Assay 2 18 9.1 0.28 3
Intra-Assay 3 18 2.9 0.12 4.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E1163Mo

    $320.00

  • 96T

    E1163Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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