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Home > Product > ELISA Kit > Mouse Glucose-6-phosphate Isomerase, GPI ELISA Kit

Mouse Glucose-6-phosphate Isomerase, GPI ELISA Kit

Mouse Glucose-6-phosphate Isomerase, GPI ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.147ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.147ng/mL

Detection range: 0.3-90ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Glucose-6-Phosphate Isomerase

Code

E1378Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.147ng/mL

Detection range

0.3-90ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed: 7277315, PubMed: 2344351, PubMed: 8417789). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (By similarity). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed: 3764429, PubMed: 3352745). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed: 3352745).

UniProt accession

MASS(Da)

62,767

GeneID

14751

Synonyms

AMF;Autocrine motility factor;Glucose-6-phosphate isomerase;GPI;Neuroleukin;NLK;PGI;PHI;Phosphoglucose isomerase;Phosphohexose isomerase

Gene names

GPI

Research area

Metabolism

Target protein

GPI

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1378Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/mL 2.165 2.113 2.067
2.062
24ng/mL 1.555 1.45 1.403
1.344
12ng/mL 0.694 0.661 0.615
0.629
6ng/mL 0.409 0.432 0.385
0.455
3ng/mL 0.124 0.15 0.104
0.176
0ng/mL 0.051 0.047 0
0.042

Precision

We measured random samples of E1378Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 27.7 1.08 3.9
Intra-Assay 2 18 12.3 0.36 2.9
Intra-Assay 3 18 11.7 0.61 5.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E1378Mo

    $320.00

  • 96T

    E1378Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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