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Home > Product > ELISA Kit > Mouse Podocalyxin, PCX ELISA Kit

Mouse Podocalyxin, PCX ELISA Kit

Mouse Podocalyxin, PCX ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.23ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.23ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Podocalyxin

Code

E1453Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.23ng/ml

Detection range

0.5-100ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

UniProt accession

MASS(Da)

53,389

GeneID

27205

Synonyms

PC;PCLP-1;Podocalyxin;Podocalyxin-like protein 1;PODXL

Gene names

PODXL

Research area

Signal Transduction

Target protein

PODXL

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1453Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
64ng/ml 2.058 2.087 2.04
2.117
32ng/ml 1.116 1.062 1.015
1.009
16ng/ml 0.401 0.44 0.393
0.479
8ng/ml 0.211 0.264 0.216
0.317
4ng/ml 0.081 0.114 0.067
0.148
0ng/ml 0.05 0.048 0
0.045

Precision

We measured random samples of E1453Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 22.5 0.84 3.7
Intra-Assay 2 18 40.8 1.01 2.5
Intra-Assay 3 18 24.3 1 4.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E1453Mo

    $320.00

  • 96T

    E1453Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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