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Home > Product > ELISA Kit > Mouse Coagulation Factor 7, F7 ELISA Kit

Mouse Coagulation Factor 7, F7 ELISA Kit

Mouse Coagulation Factor 7, F7 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.116ng/mL

Detection range: 0.2-60ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.116ng/mL

Detection range: 0.2-60ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Coagulation Factor 7

Code

E1470Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.116ng/mL

Detection range

0.2-60ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium (By similarity).

UniProt accession

MASS(Da)

50,276

GeneID

14068

Synonyms

Coagulation factor VII;F7;Factor VII heavy chain;Factor VII light chain;Serum prothrombin conversion accelerator

Gene names

F7

Research area

Blood Coagulation

Target protein

F7

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1470Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/mL 2.298 2.221 2.161
2.144
16ng/mL 1.206 1.206 1.146
1.205
8ng/mL 0.654 0.726 0.666
0.797
4ng/mL 0.534 0.509 0.449
0.484
2ng/mL 0.179 0.202 0.142
0.225
0ng/mL 0.059 0.06 0
0.061

Precision

We measured random samples of E1470Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 13.8 0.68 4.9
Intra-Assay 2 18 20.5 0.72 3.5
Intra-Assay 3 18 6.9 0.45 6.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E1470Mo

    $320.00

  • 96T

    E1470Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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