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Home > Product > ELISA Kit > Mouse Peroxisome Proliferator-activated Receptor Gamma Coactivator 1 Alpha, PGC1A ELISA Kit

Mouse Peroxisome Proliferator-activated Receptor Gamma Coactivator 1 Alpha, PGC1A ELISA Kit

Mouse Peroxisome Proliferator-activated Receptor Gamma Coactivator 1 Alpha, PGC1A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.33ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 7.33ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1 Alpha

Code

E1487Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.33ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK. Isoform 4 specifically activates the expression of IGF1 and suppresses myostatin expression in skeletal muscle leading to muscle fiber hypertrophy.

UniProt accession

MASS(Da)

90,588

GeneID

19017

Synonyms

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha;PGC-1-alpha;PPAR-gamma coactivator 1-alpha;PPARGC 1A;PPARGC1A;PPARGC-1-alpha

Gene names

PPARGC1A

Research area

Metabolism

Target protein

PPARGC1A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1487Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.027 2.179 2.113
2.33
800ng/L 1.338 1.4 1.334
1.462
400ng/L 0.923 0.905 0.839
0.887
200ng/L 0.453 0.467 0.401
0.481
100ng/L 0.293 0.273 0.208
0.253
0ng/L 0.072 0.066 0
0.059

Precision

We measured random samples of E1487Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1125.5 44.79 4
Intra-Assay 2 18 425.8 22.15 5.2
Intra-Assay 3 18 148.4 8.03 5.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E1487Mo

    $320.00

  • 96T

    E1487Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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