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Home > Product > ELISA Kit > Mouse Protein Wnt-5A, WNT5AELISA Kit

Mouse Protein Wnt-5A, WNT5AELISA Kit

Mouse Protein Wnt-5A, WNT5AELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Protein Wnt-5A

Code

E1496Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.021ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Ligand for members of the frizzled family of seven transmembrane receptors (PubMed: 17117926). Can activate or inhibit canonical Wnt signaling, depending on receptor context (PubMed: 16602827). In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (PubMed: 16602827). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration (PubMed: 17117926). Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells (By similarity). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle (PubMed: 10021340). Inhibits type II collagen expression in chondrocytes (By similarity).

UniProt accession

MASS(Da)

42,309

GeneID

22418

Synonyms

Protein Wnt-5a;WNT 5A;WNT5A

Gene names

WNT5A

Research area

Stem Cells

Target protein

WNT5A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1496Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12.8ng/ml 2.007 2.029 2
2.051
6.4ng/ml 1.12 1.099 1.069
1.077
3.2ng/ml 0.517 0.537 0.507
0.557
1.6ng/ml 0.232 0.239 0.209
0.245
0.8ng/ml 0.17 0.126 0.096
0.081
0ng/ml 0.029 0.029 0
0.029

Precision

We measured random samples of E1496Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.4 0.31 4.8
Intra-Assay 2 18 10.7 0.35 3.3
Intra-Assay 3 18 2.9 0.15 5.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E1496Mo

    $320.00

  • 96T

    E1496Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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