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Home > Product > ELISA Kit > Mouse Monocarboxylate Transporter-1, MCT-1 ELISA Kit

Mouse Monocarboxylate Transporter-1, MCT-1 ELISA Kit

Mouse Monocarboxylate Transporter-1, MCT-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.103ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.103ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Monocarboxylate Transporter-1

Code

E1525Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.103ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.

UniProt accession

MASS(Da)

53,267

GeneID

20501

Synonyms

MCT 1;Monocarboxylate transporter 1;SLC16A1;Solute carrier family 16 member 1

Gene names

SLC16A1

Research area

Signal Transduction

Target protein

SLC16A1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1525Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.137 2.204 2.169
2.272
16ng/ml 1.204 1.238 1.202
1.272
8ng/ml 0.707 0.685 0.65
0.664
4ng/ml 0.377 0.382 0.346
0.387
2ng/ml 0.131 0.133 0.097
0.135
0ng/ml 0.026 0.036 0
0.046

Precision

We measured random samples of E1525Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.4 0.34 5.4
Intra-Assay 2 18 18 0.56 3.1
Intra-Assay 3 18 11.8 0.65 5.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E1525Mo

    $320.00

  • 96T

    E1525Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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