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Home > Product > ELISA Kit > Mouse Sestrin 2, SESN2 ELISA Kit

Mouse Sestrin 2, SESN2 ELISA Kit

Mouse Sestrin 2, SESN2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Sestrin 2

Code

E1534Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.027ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed: 18692468, PubMed: 25259925). This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. May negatively regulate protein translation in response to endoplasmic reticulum stress, via TORC1 (PubMed: 24947615). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed: 23274085). May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress (PubMed: 18692468). Has an alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (By similarity). However, this could not be confirmed (By similarity).

UniProt accession

MASS(Da)

54,377

GeneID

230784

Synonyms

SESN 2;SESN2;Sestrin-2

Gene names

SESN2

Research area

Cardiovascular

Target protein

SESN2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1534Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.118 2.005 1.966
1.891
8ng/ml 1.051 1.054 1.016
1.057
4ng/ml 0.613 0.605 0.567
0.597
2ng/ml 0.301 0.267 0.229
0.234
1ng/ml 0.12 0.128 0.09
0.137
0ng/ml 0.027 0.038 0
0.05

Precision

We measured random samples of E1534Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 9.3 0.52 5.6
Intra-Assay 2 18 14.3 0.47 3.3
Intra-Assay 3 18 4.8 0.28 5.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E1534Mo

    $320.00

  • 96T

    E1534Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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