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Home > Product > ELISA Kit > Mouse Protein Wnt-3A, WNT3A ELISA Kit

Mouse Protein Wnt-3A, WNT3A ELISA Kit

Mouse Protein Wnt-3A, WNT3A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.65ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 7.65ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Protein Wnt-3A

Code

E1565Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.65ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed: 26902720). Required for normal embryonic mesoderm development and formation of caudal somites (PubMed: 8299937). Required for normal morphogenesis of the developing neural tube (PubMed: 8299937). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed: 26902720).

UniProt accession

MASS(Da)

39,258

GeneID

22416

Synonyms

Protein Wnt-3a;WNT 3A;WNT3A

Gene names

WNT3A

Research area

Stem Cells

Target protein

WNT3A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1565Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.128 2.216 2.174
2.305
800ng/L 1.649 1.673 1.631
1.697
400ng/L 1.066 1.058 1.016
1.051
200ng/L 0.69 0.676 0.634
0.662
100ng/L 0.397 0.419 0.377
0.441
0ng/L 0.049 0.042 0
0.035

Precision

We measured random samples of E1565Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 664.9 29.98 4.5
Intra-Assay 2 18 476.1 36.82 7.7
Intra-Assay 3 18 86.9 4.56 5.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E1565Mo

    $320.00

  • 96T

    E1565Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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