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Home > Product > ELISA Kit > Mouse A Disintegrin and Metalloproteinase with Thrombospondin Motifs 5, ADAMTS5 ELISA Kit

Mouse A Disintegrin and Metalloproteinase with Thrombospondin Motifs 5, ADAMTS5 ELISA Kit

Mouse A Disintegrin and Metalloproteinase with Thrombospondin Motifs 5, ADAMTS5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.113ng/mL

Detection range: 0.2-70ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.113ng/mL

Detection range: 0.2-70ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

A Disintegrin and Metalloproteinase with Thrombospondin Motifs 5

Code

E1580Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.113ng/mL

Detection range

0.2-70ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Metalloproteinase that plays an important role in connective tissue organization, development, inflammation and cell migration. Extracellular matrix (ECM) degrading enzyme that shows proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN and NCAN. Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its VCAN remodeling properties. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration (PubMed: 23233679). Participates in the development of brown adipose tissue and browning of white adipose tissue (PubMed: 28702327). Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection (PubMed: 27855162).

UniProt accession

MASS(Da)

101,844

GeneID

23794

Synonyms

A disintegrin and metalloproteinase with thrombospondin motifs 5;ADAMTS 5;ADAM-TS 5;ADAMTS5;ADAMTS-5;ADAM-TS5;ADMP-2;Aggrecanase-2;Implantin;

Gene names

ADAMTS5

Research area

Cell Biology

Target protein

ADAMTS5

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1580Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/mL 2.37 2.244 2.207
2.118
20ng/mL 1.291 1.286 1.249
1.282
10ng/mL 0.747 0.729 0.692
0.712
5ng/mL 0.346 0.357 0.32
0.369
2.5ng/mL 0.149 0.174 0.137
0.198
0ng/mL 0.036 0.037 0
0.038

Precision

We measured random samples of E1580Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6 0.26 4.4
Intra-Assay 2 18 22.1 0.71 3.2
Intra-Assay 3 18 17.6 1 5.7

Protocols

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  • Size

    Code

    Price

  • 48T

    E1580Mo

    $320.00

  • 96T

    E1580Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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