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Home > Product > ELISA Kit > Mouse Estradiol 17-beta-dehydrogenase 2, HSD17B2 ELISA Kit

Mouse Estradiol 17-beta-dehydrogenase 2, HSD17B2 ELISA Kit

Mouse Estradiol 17-beta-dehydrogenase 2, HSD17B2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.043ng/ml

Detection range: 0.1-35ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.043ng/ml

Detection range: 0.1-35ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Estradiol 17-Beta-Dehydrogenase 2

Code

E1693Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.043ng/ml

Detection range

0.1-35ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.

UniProt accession

MASS(Da)

41,836

GeneID

15486

Synonyms

17-beta-HSD 2;17-beta-hydroxysteroid dehydrogenase type 2;Estradiol 17-beta-dehydrogenase 2;HSD 17B2;HSD17B2;Testosterone 17-beta-dehydrogenase

Gene names

HSD17B2

Research area

Signal Transduction

Target protein

HSD17B2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1693Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/ml 1.813 1.914 1.88
2.016
10ng/ml 1.101 0.988 0.954
0.874
5ng/ml 0.42 0.429 0.395
0.438
2.5ng/ml 0.169 0.169 0.135
0.17
1.25ng/ml 0.09 0.106 0.072
0.122
0ng/ml 0.026 0.034 0
0.042

Precision

We measured random samples of E1693Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.2 0.18 3.4
Intra-Assay 2 18 15.8 0.45 2.8
Intra-Assay 3 18 11.1 0.48 4.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E1693Mo

    $320.00

  • 96T

    E1693Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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