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Home > Product > ELISA Kit > Mouse Aminopeptidase N, ANPEP ELISA Kit

Mouse Aminopeptidase N, ANPEP ELISA Kit

Mouse Aminopeptidase N, ANPEP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.97mU/mL

Detection range: 20-3500mU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.97mU/mL

Detection range: 20-3500mU/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Aminopeptidase N

Code

E1834Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.97mU/mL

Detection range

20-3500mU/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Broad specificity aminopeptidase which plays a role in the final digestion of Peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various Peptides including Peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines (By similarity). May also be involved the cleavage of Peptides bound to major histocompatibility complex class II molecules of antigen presenting cells (PubMed: 8691132). May have a role in angiogenesis and promote cholesterol crystallization (By similarity). May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (PubMed: 22677001).

UniProt accession

MASS(Da)

109,651

GeneID

16790

Synonyms

Alanyl aminopeptidase;Aminopeptidase M;Aminopeptidase N;ANPEP;AP-M;AP-N;CD antigen CD13;mAPN;Membrane protein p161;Microsomal aminopeptidase

Gene names

ANPEP

Research area

Cardiovascular

Target protein

ANPEP

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1834Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000mU/mL 2.267 2.201 2.167
2.134
1000mU/mL 1.54 1.646 1.613
1.753
500mU/mL 1.111 1.118 1.085
1.125
250mU/mL 0.7 0.709 0.675
0.718
125mU/mL 0.447 0.465 0.431
0.482
0mU/mL 0.04 0.033 0
0.027

Precision

We measured random samples of E1834Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 321 24.13 7.5
Intra-Assay 2 18 811.4 41.61 5.1
Intra-Assay 3 18 244.8 19.07 7.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E1834Mo

    $320.00

  • 96T

    E1834Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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