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Home > Product > ELISA Kit > Mouse Beta-type Platelet-derived Growth Factor Receptor, PDGFRB ELISA Kit

Mouse Beta-type Platelet-derived Growth Factor Receptor, PDGFRB ELISA Kit

Mouse Beta-type Platelet-derived Growth Factor Receptor, PDGFRB ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.36ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 7.36ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Beta-Type Platelet-Derived Growth Factor Receptor

Code

E1881Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.36ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM (By similarity). Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

UniProt accession

MASS(Da)

122,790

GeneID

18596

Synonyms

Beta platelet-derived growth factor receptor;Beta-type platelet-derived growth factor receptor;CD antigen CD140b;CD140 antigen-like family member B;PDGFR-1;PDGFRB;PDGFR-beta;PDGF-R-beta;Platelet-derived growth factor receptor 1;Platelet-derived growth factor receptor beta

Gene names

PDGFRB

Research area

Signal Transduction

Target protein

PDGFRB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1881Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 1.809 1.819 1.769
1.829
800ng/L 0.91 0.943 0.894
0.976
400ng/L 0.505 0.552 0.502
0.6
200ng/L 0.17 0.234 0.184
0.298
100ng/L 0.114 0.128 0.078
0.143
0ng/L 0.061 0.05 0
0.039

Precision

We measured random samples of E1881Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 668.2 23.03 3.4
Intra-Assay 2 18 1216.9 38.27 3.1
Intra-Assay 3 18 373.3 23.3 6.2

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E1881Mo

    $320.00

  • 96T

    E1881Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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