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Home > Product > ELISA Kit > Mouse C-C Chemokine Receptor Type 2, CCR2 ELISA Kit

Mouse C-C Chemokine Receptor Type 2, CCR2 ELISA Kit

Mouse C-C Chemokine Receptor Type 2, CCR2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.49ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.49ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

C-C Chemokine Receptor Type 2

Code

E1916Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.49ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Key functional receptor for CCL2 but can also bind CCL7 and CCL12 chemokines (PubMed: 8631787, PubMed: 8662823, PubMed: 8996246). Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (By similarity). Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B (By similarity). Regulates the expression of T-cell inflammatory cytokines and T-cell differentiation, promoting the differentiation of T-cells into T-helper 17 cells (Th17) during inflammation (PubMed: 28507030). Facilitates the export of mature thymocytes by enhancing directional movement of thymocytes to sphingosine-1-phosphate stimulation and up-regulation of S1P1R expression; signals through the JAK-STAT pathway to regulate FOXO1 activity leading to an increased expression of S1P1R (PubMed: 29930553). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (PubMed: 29993042). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (PubMed: 29993042). Mediates the recruitment of macrophages and monocytes to the injury site following brain injury (PubMed: 24806994, PubMed: 29632244).

UniProt accession

MASS(Da)

42,783

GeneID

12772

Synonyms

C-C chemokine receptor type 2;C-C CKR-2;CC-CKR-2;CCR 2;CCR2;CCR-2;CD antigen CD192;JE/FIC receptor;MCP-1 receptor;

Gene names

CCR2

Research area

Immunology

Target protein

CCR2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1916Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.155 2.139 2.085
2.123
600ng/L 1.042 1.082 1.028
1.123
300ng/L 0.424 0.464 0.409
0.503
150ng/L 0.222 0.203 0.149
0.184
75ng/L 0.125 0.138 0.084
0.152
0ng/L 0.048 0.054 0
0.06

Precision

We measured random samples of E1916Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 684.5 27.89 4.1
Intra-Assay 2 18 914.2 25.88 2.8
Intra-Assay 3 18 257.1 10.62 4.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E1916Mo

    $320.00

  • 96T

    E1916Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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