Product overview
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Background
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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Components
Components | Quantity |
---|---|
Pre-coated plate | 12 * 8 well strips x 1 |
Positive control | 0.5ml x 1 vial |
Negative control | 0.5ml x 1 vial |
HRP conjugated | 6ml x 1 vial |
Sample diluent | 6ml x 1 vial |
Substrate solution A | 6ml x1 vial |
Substrate solution B | 6ml x1 vial |
Stop solution | 6ml x1 vial |
Wash buffer (25x) | 20ml x 1 vial |
Protocol data
These standard curves of E3339Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
Precision
We measured random samples of E3339Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Protocols
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